site stats

Ctcf kda

WebAug 17, 2024 · CTCF is required for peripheral nerve ensheathment a Excised exon 8 of the floxed Ctcf allele by Dhh-Cre. b Co-labeling of CTCF with SOX10 in control and mutant sciatic nerves at P7 (n = 3 animals ... WebThe recombinant protein with a predicted molecular weight of 43.3 kDa was removed from the gel and purified by electroelution. The electroelution was conducted in cellulose tubes (Sanko Junyaku Co., Ltd., Tokyo, Japan) filled with an electroelution buffer (100 mM sodium phosphate and 0.1% SDS; pH 7.0) at 100 V and 100 mA for 17 h.

CTCF loss has limited effects on global genome architecture in

WebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific … WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. richeson color wheel https://ptforthemind.com

CTCF: an architectural protein bridging genome topology

WebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. WebHome; Products. Featured Collections. Recombinant Rabbit Monoclonal Antibodies; IHC/Pathology Antibodies; KO Validated Antibodies; Antibodies. Primary Antibodies WebThe Division of Data Processing is headed by a Chief of Data Processing and is responsible for the development, utilization and coordination of the Department's Data computer … red original active jacket

The molecular weight determination of CTCF protein …

Category:CTCF: an architectural protein bridging genome topology …

Tags:Ctcf kda

Ctcf kda

The 70 kDa protein is a truncated version of the 130 kDa …

WebFamily First is an opportunity to provide enhanced support to children and families. It aims to prevent foster care placements through the provision of mental health and substance … WebJun 8, 2004 · The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5' end of the chicken beta-globin locus. ... a 38-kDa nucleolar phosphoprotein that is concentrated in nuclear matrix preparations. In this ...

Ctcf kda

Did you know?

WebThis 70 kDa band for CTCF has been described previously [197,245] as a possible N-terminally truncated CTCF protein generated by alternative translation of CTCF mRNA which is known to enhance CTCF ... Web17.9 kDa No: 0: CTCF-212 ENSP00000495218 ENST00000645306: A0A2R8YFL0 [Direct mapping] Transcriptional repressor CTCF . Show all. Predicted intracellular proteins Intracellular proteins predicted by MDM and MDSEC Cancer-related genes Mutated cancer genes Mutational cancer driver genes

WebMay 1, 2006 · Interestingly, the 180 kDa poly(ADP-ribosyl)ated isoform of CTCF was predominantly found in the nucleoli fractions. By transfecting different CTCF deletion constructs into cell lines of different origin we demonstrate that the central zinc-finger domain of CTCF is the region responsible for nucleolar targeting. Analysis of … WebApr 10, 2024 · It contains 37 exons that encode a 2492 amino acid protein with a molecular weight of 282.586 kDa [22,23]. Based on its ATPase/helicase C-terminal domain, the protein is a member of the SWI/SNF family. ... (CTCF, which is localized by H3.3 histones) and boundary disturbances of the chromatin loop, which promote coordinated loop-wide …

WebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human … WebJan 7, 2024 · For example, CTCF re-localizes to the nucleolus during growth arrest in apoptotic and differentiated cells in several experimental systems. Interestingly, the 180 kDa poly(ADP-ribosyl)ated isoform of CTCF is targeted to the nucleolus through its central zinc finger domain where it appears to inhibit RNA polymerase I-dependent transcription .

WebMay 23, 2016 · The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We …

WebProceedings of the National Academy of Sciences of the United States of ... red original releaseWebMay 1, 2024 · The loss of CTCF poly ADP-ribosyltion in breast cancer leading to the expression of both 180-kDa and 130-kDa in comparison to only 180-kDa CTCF in normal breast tissues likely ... 2009). Although CTCF-130-kDa or Rb2/p130 can be used as the biomarker for the cancer progression, the utility of CTCF-130-kDa as the disease … richeson flat fileWebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific targets of poly(ADP-ribosyl)ation in tumor development. Two forms of the multifunctional transcription factor, CTCF, were previously identified: a 130-kDa protein (CTCF-130), … richeson etching press