WebFluoroFix™ is a gentler fixation PFA-based buffer. Cyto-Fast™ Fix/Perm Buffer Set is a specialized buffer for cytokine detection and offers improved staining over the Intracellular Staining Permeabilization Wash Buffer. True-Nuclear™ Transcription Factor Buffer Set is designed for optimal staining of intranuclear targets. WebDec 30, 2024 · Dulbecco’s Phosphate Buffered Saline (DPBS, #1404-133) was purchased from ThermoFisher Scientific. Cell Stimulation 500 \times Cocktail (a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin, Catalog# 00 …
Flow Buffers - BioLegend
WebTip: For gentler fixation (particularly with tandem fluors), FluoroFix™ Buffer (Cat. No. 422101) may be used. Perform fluorescence activated cell sorting (FACS), or flow cytometric analysis. Note: If you are unable to immediately read your samples on a cytometer, keep them shielded from light and in a refrigerator set at 4-8°C. WebDec 7, 2016 · Pelleted cells were resuspended in FACS buffer (PBS, 2 mM EDTA, and 5% fetal bovine serum) for immunos-taining and the subsequent flow cytometric analyses. ... CA, USA). Samples were fixed in FluoroFix buffer (BioLegend, San Diego, CA, USA) and stored at 4°C until analysis. For intracellular staining, cells were fixed and permeabilized … city bike pricelist
Fixation Buffer - Sony Biotechnology
WebFluoroFix™ is a gentler fixation PFA-based buffer. Cyto-Fast™ Fix/Perm Buffer Set is a specialized buffer for cytokine detection and offers improved staining over the Intracellular Staining Permeabilization Wash Buffer. … WebAs such, Stain Buffer (FBS) is designed to maintain cell viability and maximize fluorescence signal intensities generated by pH-sensitive fluorochromes, e.g., fluoresceine isothiocyanate (FITC). In addition, Stain Buffer (FBS) contains the … WebDec 23, 2024 · Mix 1 part of 10× Permeabilization Buffer with 9 parts of distilled water. For example, mix 5 mL 10× Permeabilization Buffer with 45 mL distilled water. This solution should be prepared on the day of sample processing and stored at … dick\u0027s athletic wear