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Grinch cells

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Upscaling of hiPS Cell–Derived Neurons for High-Throughput …

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Grinch cells

Membrane order regulates SNARE mediated vesicle fusion in …

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Grinch cells

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WebMar 1, 2024 · GRINCH cells displayed neuronal markers, and their functional activity could be demonstrated by electrophysiological recordings. In an application of GRINCH neurons, the brain-derived neurotrophic factor (BDNF)–mediated activation of tropomyosin receptor kinase (TrkB) was investigated as a promising drug target to treat synaptic dysfunctions. WebAug 18, 2024 · reagents including the GRINCH cells. J.D.C. wrote the manuscript and M.T.H. organized the figures and incorporated statistical analyses. †These authors contributed equally to the reported studies. ‡Present address: Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205.

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WebFeb 11, 2024 · Cells enriched with saturated lipids showed a decrease in vesicle fusion efficiency, while cells enriched with unsaturated lipids showed an increase in fusion efficiency. Experiments with supported membranes containing recombinant SNARE proteins and granules purified from INS1-derived GRINCH cells confirmed that lipid acyl chain …

WebJul 3, 2024 · Cells were incubated at 37 °C, in humidified 95% air and 5% CO 2, and passaged every 3–4 days using TrypLE Express for 3 min at 37 °C. All cells were tested mycoplasma-negative, except for GRINCH cells used in preliminary data. All cell culture reagents were from Life Technologies, Inc. Adenovirus syncollin-dsRedE5TIMER infection good morning america pastinaWebSep 5, 2024 · GRINCH cells have inserted GFP into the human C-peptide insulin gene. Hence, secreted CpepGFP served to monitor insulin secretion. mRNA silencing of FTO … good morning america paula faris mother dayWebNov 9, 2024 · ( A) Flow chart for purifying insulin granules from INS 1-derived (GRINCH or INS 832/13) cells over a density gradient, assessing their syt content, and further … chess after work gent