NettetUntreated adult Mpl −/− and Tpo Tg mice had normal lymphocyte and WBC numbers (supplemental Figure 2B-C) and spleen size. 7, 32, 33 We first compared the WT and Mpl −/− recipients, where Eμ-myc lymphoma progression was reduced in thrombocytopenic Mpl −/− mice compared than in WT mice, based on reduced WBC and lymphocyte … NettetThis application protocol describes the flow cytometric analysis of dendritic cells (DCs) after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily …
Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic ...
Nettetin human and mouse samples Major cell populations Adult human blood (typical number/L) Human PBMC* (typical number/L) Mouse spleen (typical number/spleen) Mouse lymph nodes Red blood cells ~5 x 1012 NA † ~2.5–5 x 1010 Variable Platelets ~3 x 1011 NA † NA † NA † White blood cells ~7 x 109 ~1–2 x 109 per L human blood ~1 x … Nettet1. jan. 2006 · Uptake of pRBC by splenic DC subsets was determined by staining CD11c + cells cocultured with pRBCs with PE-labeled mAb to B220 (clone R43-6B2), CD4 (clone GK1.5), and CD8 (clone 53.6.7) ... freshly purified from naive B6 mice, in varying ratios as indicated at a final volume of 200 μl in 96-well plates for 48 h at 37°C. crescent head tavern pizza
Isolation of Mouse Spleen Dendritic Cells - Springer
Nettet15. des. 2000 · Three distinct subtypes of dendritic cells (DC) are present in mouse spleen, separable as CD4 (-)8alpha (-), CD4 (+)8alpha (-), and CD4 (-)8alpha (+) DC. … Nettet10 Stagg et al. can both activate resting T cells, but may stimulate different types of responses. The CD8__+ population has been reported to drive preferentially Th1 … NettetThis study reports on a method for the propagation of large numbers of dendritic cells (DC) from mouse spleen using granulocyte-macrophage colony-stimulating factor (GM-CSF) and their characteristics. Within a few days of liquid culture in GM-CSF, B10 BR (H-2k, I-E+) mouse splenocytes formed loosely adherent myeloid cell clusters. crescent health centre