網頁2024年4月12日 · When the cell fusion degree reached 80–90%, the cells were subcultured and digested with 0.25% trypsin solution. The cells were used in the experiment after 3 passages. The cells were resuspended in a complete medium to a 5 × 10 4 /mL concentration and inoculated into 96-well plates. 網頁2024年4月13日 · Human blood mononuclear cells were cultured, and cytokine release was determined by ELISA. Cell proliferation ability was assessed using the MTT assay. Compared with the control group, the mRNA and protein expression levels of tumor necrosis factor‑α (TNF‑α) were downregulated in tumor tissues and serum and the serum levels of …
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http://www.cckn-ia.org/2024/09/19/%ef%bb%bfwhen-the-cells-reach-90-confluence-the-cells-were-lifted-with-0/ 網頁The proteins in dried gel pieces were digested with trypsin by incubation with 20 μL of trypsin solution (10 ng trypsin in 50% ACN/10 mM ammonium bicarbonate) at RT for 20 min. Then 30 μL of 30% ACN was added to immerse the gels throughout the digestion process and left overnight at RT. divergent herald of purity
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網頁2013年10月30日 · Yeast total protein extract was digested overnight with trypsin or Trypsin/Lys-C Mix. The digests were analyzed using an Orbitrap Velos mass … 網頁Five digestion settings were performed in parallel with chymotrypsin, Lys-C, trypsin, V8-DE and V8-E. In this experiment, the number of missed cleavages observed was highly variable.... 網頁cas. sol. + 20 c.c. H20, were digested for 1 day and 4 days. Thecontrol digested 1 day gave Ncorresp. to 0-75 c.c. N.V acid. 4 days,, ,, 140 Byinterpolation one obtains: for 2 days 0-95-3,, 1-20 Thenon-precipitable nitrogen in 10 c.c. of trypsin was found to From cracked monster party