WebTthSSB appears to be positioned near the center of the (dT)60+n ssDNA overhangs though rapid diffusion. (A) A schematic illustration of our experimental design for (dT)60+n (n = 0, … http://www.100md.com/html/DirDu/2006/07/27/12/18/65.htm
Novel thermostable single-stranded DNA-binding protein (SSB) …
WebA fixed quantity (20 pmol) of 5h-end fluorescein-labelled oligonucleotides (dT) 30 , (dT) 60 , (dT) 70 or (dT) 76 was incubated with 0, 5, 20, 50 and 200 pmol of TthSSB in 20 µl … Webfrom T.thermophilus strain HB8 (TthSSB) increases the effi-*To whom correspondence should be addressed. Tel: +81 45 508 7224; Fax: +81 45 508 7364; Email: [email protected] The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors The Author 2006. Published by Oxford University Press. cyngor cymuned llanberis
Characterization of exceptionally thermostable single-stranded …
WebMay 12, 2009 · Use of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500x. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over … WebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over linear targets. Crown", WebAug 8, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand billy markus photo